文獻名： A magnetic relaxation switch sensor for determination of 17βEstradiol in milk and eggs based on aptamerfunctionalized Fe3O4@Au nanoparticles
作者： Xin Wang, Kaili Pei, Hanying Sun, Qi Wang
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China
摘要：
BACKGROUND
A simple and rapid detection method for 17β-estradiol (E2) in complex food matrix is greatly desirable. A magnetic relaxation switch (MRS) sensor for detecting E2 based on the aptamer-functionalized gold-coated iron oxide (Fe3O4@Au) nanocomposite was designed in this study. Fe3O4@Au nanoparticles (NPs) played as a ‘switch’ between dispersed and aggregated states, while aptamer served as the recognition unit.
RESULTS
According to the sensing effect of monocomponent relaxation time (T2W) for E2, the volume ratio of aptamers to Fe3O4@Au, the sodium chloride (NaCl) concentration, the concentration of Fe3O4@Au@Apt, and reaction time were optimized to be 4:1, 0.03 mol L–1, 4 μmol L–1 and 15 min, respectively. For the analysis of food sample, the E2 was quantified over a concentration range of 1 to 100 nmol L–1 with a detection limit of 7.6 nmol L–1 for milk samples, while a linearity range of 20 to 100 nmol L–1 and a detection limit of 8.57 nmol L–1 for egg samples.
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